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primary human newborn foreskin fibroblasts nuff-1 cells  (GlobalStem)

 
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    GlobalStem primary human newborn foreskin fibroblasts nuff-1 cells
    Primary Human Newborn Foreskin Fibroblasts Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human newborn foreskin fibroblasts nuff-1 cells/product/GlobalStem
    Average 90 stars, based on 1 article reviews
    primary human newborn foreskin fibroblasts nuff-1 cells - by Bioz Stars, 2026-03
    90/100 stars

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    primary human newborn foreskin fibroblasts nuff-1 cells  (GlobalStem)
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    GlobalStem primary human newborn foreskin fibroblasts nuff-1 cells
    Primary Human Newborn Foreskin Fibroblasts Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A. & B. Analysis of the impact of PALA co-treatment on ISG transcripts induced by IFNβ by qRT-PCR. Mx1, Isg15, and Ifit1 ( A. ) or Ifit2 and Oas1 ( B. ) transcript levels in Raw264.7 cells treated with IFNβ (200u/mL), PALA (100uM), or co-stimulation with IFNβ and PALA for 24h (n=4 experiments). Data presented as mean±SEM; significance determined by one-way ANOVA and Tukey’s multiple comparisons test; ns=not significant, *** p≤0.001 vs. NT, ++ p≤0.01 vs. IFN. C. PALA suppresses HCMV replication. ARPE-19 cells were infected with HCMV (MOI=3 TCID 50 /cell) and then cultured in media ±PALA (100μM). De novo cell-associated virus production was titered by TCID 50 assay on naïve <t>NuFF-1</t> cells (n=3 experiments performed in triplicate; representative experiment shown). Data presented as mean±SEM; significance determined by one-way ANOVA and Sidak’s multiple comparisons test; ns=not significant, *p≤0.05, **p≤0.01 media vs. PALA; #p≤0.05, ##p≤0.01 vs. 24h sample. D. ARPE-19 cells were infected (MOI=3 TCID 50 /cell) and lysates collected at the indicated times post-infection. Expression of viral proteins were assessed by immunoblot probed with anti-IE1, anti-pUL44, and anti-tubulin (n=3 experiments; representative experiment shown).
    Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    newborn human foreskin fibroblast-1 (nuff) cells  (GlobalStem)
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    A. & B. Analysis of the impact of PALA co-treatment on ISG transcripts induced by IFNβ by qRT-PCR. Mx1, Isg15, and Ifit1 ( A. ) or Ifit2 and Oas1 ( B. ) transcript levels in Raw264.7 cells treated with IFNβ (200u/mL), PALA (100uM), or co-stimulation with IFNβ and PALA for 24h (n=4 experiments). Data presented as mean±SEM; significance determined by one-way ANOVA and Tukey’s multiple comparisons test; ns=not significant, *** p≤0.001 vs. NT, ++ p≤0.01 vs. IFN. C. PALA suppresses HCMV replication. ARPE-19 cells were infected with HCMV (MOI=3 TCID 50 /cell) and then cultured in media ±PALA (100μM). De novo cell-associated virus production was titered by TCID 50 assay on naïve <t>NuFF-1</t> cells (n=3 experiments performed in triplicate; representative experiment shown). Data presented as mean±SEM; significance determined by one-way ANOVA and Sidak’s multiple comparisons test; ns=not significant, *p≤0.05, **p≤0.01 media vs. PALA; #p≤0.05, ##p≤0.01 vs. 24h sample. D. ARPE-19 cells were infected (MOI=3 TCID 50 /cell) and lysates collected at the indicated times post-infection. Expression of viral proteins were assessed by immunoblot probed with anti-IE1, anti-pUL44, and anti-tubulin (n=3 experiments; representative experiment shown).
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    A. & B. Analysis of the impact of PALA co-treatment on ISG transcripts induced by IFNβ by qRT-PCR. Mx1, Isg15, and Ifit1 ( A. ) or Ifit2 and Oas1 ( B. ) transcript levels in Raw264.7 cells treated with IFNβ (200u/mL), PALA (100uM), or co-stimulation with IFNβ and PALA for 24h (n=4 experiments). Data presented as mean±SEM; significance determined by one-way ANOVA and Tukey’s multiple comparisons test; ns=not significant, *** p≤0.001 vs. NT, ++ p≤0.01 vs. IFN. C. PALA suppresses HCMV replication. ARPE-19 cells were infected with HCMV (MOI=3 TCID 50 /cell) and then cultured in media ±PALA (100μM). De novo cell-associated virus production was titered by TCID 50 assay on naïve <t>NuFF-1</t> cells (n=3 experiments performed in triplicate; representative experiment shown). Data presented as mean±SEM; significance determined by one-way ANOVA and Sidak’s multiple comparisons test; ns=not significant, *p≤0.05, **p≤0.01 media vs. PALA; #p≤0.05, ##p≤0.01 vs. 24h sample. D. ARPE-19 cells were infected (MOI=3 TCID 50 /cell) and lysates collected at the indicated times post-infection. Expression of viral proteins were assessed by immunoblot probed with anti-IE1, anti-pUL44, and anti-tubulin (n=3 experiments; representative experiment shown).
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    newborn human foreskin fibroblasts nuff-1 cells  (GlobalStem)
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    GlobalStem newborn human foreskin fibroblasts nuff-1 cells
    A. & B. Analysis of the impact of PALA co-treatment on ISG transcripts induced by IFNβ by qRT-PCR. Mx1, Isg15, and Ifit1 ( A. ) or Ifit2 and Oas1 ( B. ) transcript levels in Raw264.7 cells treated with IFNβ (200u/mL), PALA (100uM), or co-stimulation with IFNβ and PALA for 24h (n=4 experiments). Data presented as mean±SEM; significance determined by one-way ANOVA and Tukey’s multiple comparisons test; ns=not significant, *** p≤0.001 vs. NT, ++ p≤0.01 vs. IFN. C. PALA suppresses HCMV replication. ARPE-19 cells were infected with HCMV (MOI=3 TCID 50 /cell) and then cultured in media ±PALA (100μM). De novo cell-associated virus production was titered by TCID 50 assay on naïve <t>NuFF-1</t> cells (n=3 experiments performed in triplicate; representative experiment shown). Data presented as mean±SEM; significance determined by one-way ANOVA and Sidak’s multiple comparisons test; ns=not significant, *p≤0.05, **p≤0.01 media vs. PALA; #p≤0.05, ##p≤0.01 vs. 24h sample. D. ARPE-19 cells were infected (MOI=3 TCID 50 /cell) and lysates collected at the indicated times post-infection. Expression of viral proteins were assessed by immunoblot probed with anti-IE1, anti-pUL44, and anti-tubulin (n=3 experiments; representative experiment shown).
    Newborn Human Foreskin Fibroblasts Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/newborn human foreskin fibroblasts nuff-1 cells/product/GlobalStem
    Average 90 stars, based on 1 article reviews
    newborn human foreskin fibroblasts nuff-1 cells - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    A. & B. Analysis of the impact of PALA co-treatment on ISG transcripts induced by IFNβ by qRT-PCR. Mx1, Isg15, and Ifit1 ( A. ) or Ifit2 and Oas1 ( B. ) transcript levels in Raw264.7 cells treated with IFNβ (200u/mL), PALA (100uM), or co-stimulation with IFNβ and PALA for 24h (n=4 experiments). Data presented as mean±SEM; significance determined by one-way ANOVA and Tukey’s multiple comparisons test; ns=not significant, *** p≤0.001 vs. NT, ++ p≤0.01 vs. IFN. C. PALA suppresses HCMV replication. ARPE-19 cells were infected with HCMV (MOI=3 TCID 50 /cell) and then cultured in media ±PALA (100μM). De novo cell-associated virus production was titered by TCID 50 assay on naïve NuFF-1 cells (n=3 experiments performed in triplicate; representative experiment shown). Data presented as mean±SEM; significance determined by one-way ANOVA and Sidak’s multiple comparisons test; ns=not significant, *p≤0.05, **p≤0.01 media vs. PALA; #p≤0.05, ##p≤0.01 vs. 24h sample. D. ARPE-19 cells were infected (MOI=3 TCID 50 /cell) and lysates collected at the indicated times post-infection. Expression of viral proteins were assessed by immunoblot probed with anti-IE1, anti-pUL44, and anti-tubulin (n=3 experiments; representative experiment shown).

    Journal: bioRxiv

    Article Title: N-phosphonacetyl-L-aspartate enhances type I interferon anti-viral responses through activation of non-canonical NOD2 signaling

    doi: 10.1101/2022.02.08.479597

    Figure Lengend Snippet: A. & B. Analysis of the impact of PALA co-treatment on ISG transcripts induced by IFNβ by qRT-PCR. Mx1, Isg15, and Ifit1 ( A. ) or Ifit2 and Oas1 ( B. ) transcript levels in Raw264.7 cells treated with IFNβ (200u/mL), PALA (100uM), or co-stimulation with IFNβ and PALA for 24h (n=4 experiments). Data presented as mean±SEM; significance determined by one-way ANOVA and Tukey’s multiple comparisons test; ns=not significant, *** p≤0.001 vs. NT, ++ p≤0.01 vs. IFN. C. PALA suppresses HCMV replication. ARPE-19 cells were infected with HCMV (MOI=3 TCID 50 /cell) and then cultured in media ±PALA (100μM). De novo cell-associated virus production was titered by TCID 50 assay on naïve NuFF-1 cells (n=3 experiments performed in triplicate; representative experiment shown). Data presented as mean±SEM; significance determined by one-way ANOVA and Sidak’s multiple comparisons test; ns=not significant, *p≤0.05, **p≤0.01 media vs. PALA; #p≤0.05, ##p≤0.01 vs. 24h sample. D. ARPE-19 cells were infected (MOI=3 TCID 50 /cell) and lysates collected at the indicated times post-infection. Expression of viral proteins were assessed by immunoblot probed with anti-IE1, anti-pUL44, and anti-tubulin (n=3 experiments; representative experiment shown).

    Article Snippet: NuFF-1 cells (GlobalStem) were maintained in DMEM with 10% FBS, 2 mM L-glutamine, 0.1 mM non-essential amino acids, 10 mM HEPES, and 100 U/ml penicillin/streptomycin.

    Techniques: Quantitative RT-PCR, Infection, Cell Culture, Expressing, Western Blot